P-119: Comparison of Weymouth and TCM 199 for Ovine Oocyte Maturation

Background: Different culture media are used for in vitro maturation of ovine oocyte. The Weymouth medium are used for in vitro maturation of rat and mice oocytes with very low levels of supplementation (Just FBS, while to acquire the good results in TCM hormone, and serum supplementation and in some cases growth factor are necessary to gain the optimized results. The aim of the present study was to compare the Weymouth and TCM199 media for maturation of ovine oocytes in vitro. Materials and Methods: The ovine ovaries were collected from the local abattoir and transported to the laboratory in saline supplemented with required antibiotics. The oocytes were aspirated, qualified and subjected to Weymouth with 10% FBS (n= 110) or TCM FSH (2.5 IU/ mL; IBSA, Switzerland,) and estradiol (30 μg/mL) and gentamycin (50 μg/mL). (n=95) in an atmosphere of 5 CO2, 95 % humidity and 38.6°C for 17-19 hours. The cultured oocytes were denuded and examined for nuclear maturation using a phase contrast microscopy. Results: The results showed a comparable efficacy of Weymouth (67.3%: 74/110) and TCM (68.4%: 65/95) in maturation of ovine oocytes. Conclusion: The Weymouth medium was efficient in maturation of ovine oocytes.